Listing all exit numbers for Hwy 401 from Windsor, through Chatham, London, Woodstock, Kitchener-Waterloo, Cambridge, Guelph, Milton, Mississauga, Toronto, Pickering, Ajax, Oshawa, Bowmanville, Newcastle, Port Hope, Cobourg, Trenton, Belleville, Napanee, Kingston, Gananoque, Brockville, Morrisburg & Cornwall to the Quebec border.
Essex Region
Hwy 3 (Start of Hwy 401)
Exit #13 – Hwy 401 (Tunnel Route)
Exit #14 – Provincial Road
Exit #21 – Manning Road
Exit #28 – Puce Road
Exit #34 – Belle River Road
Exit #40 – Essex Road 31
Exit #48 – Hwy 77
Exit #56 – Hwy 2 West
Chatham-Kent County
Exit #63 – Hwy 2 East
Merlin Road Overpass
Exit #81 – Bloomfield Road
Exit #90 – Hwy 40
Exit #101 – Kent Bridge Road
Exit #109 – Old Hwy 21
Exit #117 – Orford Road
Elgin County
Exit #129 – Furnival Road
Exit #137 – Hwy 76
Exit #149 – Currie Road
Exit #157 – Iona Road
Exit #164 – Union Road
Middlesex County
Exit #177 – Hwy 4
Exit #183 – Hwy 402
Exit #186 – Wellington Road
Exit #189 – Highbury Avenue
Exit #194 – Airport Road
Exit #195 – Hwy 74
Exit #199 – Dorchester Road
Exit #203 – Hwy 73
Exit #208 – Putnam Road
Oxford County
Exit #216 – Culloden Road
Exit #218 – Hwy 19
Exit #222 – Oxford Road 6
Exit #230 – Sweaburg Road
Exit #232 – Hwy 59
Exit #235 – Hwy 403
Exit #236 – Towerline Road
Exit #238 – Hwy 2
Exit #250 – Drumbo Road
City of Kitchener-Waterloo
Oxford Road 3 Overpass
Exit #268 – Waterloo Road 97
Exit #275 – Homer Watson Blvd
Exit #278 – Hwy 8
Exit #282 – Hwy 24
Exit #284 – Franklin Blvd
Exit #286 – Townline Road
Wellington Region
Exit #295 – Hwy 6 North
Exit #299 – Hwy 6 South
Exit #312 – Guelph Line
Exit #320 – Hwy 25
Exit #324 – James Snow Parkway
Exit #328 – Trafalgar Road
GTA Toronto
Exit #330 – Hwy 407 ETR
Exit #333 – Winston Churchill Blvd
Exit #336 – Mississauga Road
Exit #342 – Hwy 10 (Hurontario Street)
Exit #344 – Hwy 403 & Hwy 410
Exit #346 – Dixie Road
Exit #348 – Renforth Drive
Exit 351 – Carlingview Drive
Exit #352 – Hwy 427
Exit #354 – Dixon Road
Exit #355 – Hwy 409
Exit #356 – Islington Avenue
Exit #357 – Weston Road
Exit #359 – Hwy 400
Exit #362 – Keele Street
Exit #364 – Dufferin Street
Exit #365 – Allen Road
Exit #366 – Bathurst Street
Exit #367 – Hwy 11A (Avenue Road)
Exit #369 – Hwy 11 (Yonge Street)
Exit #371 – Bayview Avenue
Exit #373 – Leslie Street
Exit #375 – Hwy 404
Exit #376 – Victoria Park Avenue
Exit #378 – Warden Avenue
Exit #379 – Kennedy Road
Exit #380 – Brimley Road
Exit #381 – McCowan Road
Exit #383 – Hwy 48 (Markham Road)
Exit #385 – Neilson Road
Exit #387 – Morningside Avenue
Exit #389 – Meadowvale Road
Exit #392 – Hwy 2A & Port Union Road
City of Pickering
Exit #394 – Whites Road
Exit #397 – Liverpool Road
Exit #399 – Brock Road
Town of Ajax
Exit #401 – Westney Road
Exit #403 – Harwood Avenue
Lakeridge Road Overpass
Whitby
Exit #410 – Hwy 12 (Brock Street)
Exit #412 – Thickson Road
Oshawa
Exit #416 – Park Road
Exit #417 – Simcoe Street
Exit #418 – Ritson Road
Exit #419 – Bloor Street
Exit #425 – Courtice Road
Exit #428 – Holt Road
Exit #431 – Waverley Road
Exit #432 – Liberty Street
Exit #435 – Bennett Road
Exit #436 – Hwy 35 & Hwy 115
Exit #440 – Mill Street
Exit #448 – Newtonville Road
Exit #456 – Wesleyville Road
Port Hope
Exit #461 – Hwy 2
Exit #464 – Hwy 28
Cobourg (2 interchanges)
Exit #472 – Burnham Street
Exit #474 – Hwy 45
Exit #487 – Aird Street
Exit #497 – Percy Street
Exit #509 – Hwy 30
Northumberland Road 26 Overpass
Exit #522 – Wooler Road
Trenton
Exit #525 – Hwy 33
Exit #526 – Glen Miller Road
Belleville
Exit #538 – Wallbridge-Loyalist Road
Exit #543 – Hwy 62
Exit #544 – Hwy 37
Exit #556 – Shannonville Road
Exit #566 – Hwy 49
Exit #570 – Deseronto Road
Napanee
Exit #579 – Hwy 41
Exit #582 – Palace Road
Exit #593 – Old Hwy 133 (L & A Road 4)
Exit #599 – Wilton Road
Kingston
Exit #611 – Hwy 38
Exit #613 – Sydenham Road
Exit #615 – Sir John A. MacDonald Blvd
Exit #617 – Division Street
Exit #619 – Montreal Street
Exit #623 – Hwy 15
Exit #632 – Joyceville Road
Boundary Road Underpass
Gananoque
Exit #645 – Hwy 32
Exit #647 – 1000 Islands Parkway
Exit #648 – Hwy 2
Exit #659 – Reynolds Road
Exit #661 – Hwy 137 (Ivy Lea Bridge Approach)
Exit #675 – Malloytown Road
Exit #685 – 1000 Islands Parkway
Brockville
Exit #687 – Hwy 2
Exit #696 – Hwy 29
Exit #698 – North Augusta Road
Exit #705 – Maitland Road
Blue Church Road Overpass
Exit #716 – Edward Street
Exit #719 – North to Ottawa
Exit #721 – Hwy 16
Exit #730 – Shanly Road
L & G – S. D. & G Boundary
Exit #738 – Carman Road
Exit #750 – Hwy 31
Church Road Overpass
Exit #758 – Upper Canada Road
Exit #770 – Dickinson Road
Exit #778 – Moulinette Road
Exit #786 – Power Dam Road
Exit #789 – Hwy 138
Exit #792 – McConnell Avenue
Exit #796 – Boundary Road
Exit #804 – Summerstown Road
Exit #814 – Hwy 2 & Hwy 34
Exit #825 – Curry Hill Road
Ontario-Quebec Boundary (Hwy 401 Ends)